1FZB
CRYSTAL STRUCTURE OF CROSSLINKED FRAGMENT D
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 297 |
| Collection date | 1996-09 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.820, 95.500, 113.760 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FIBRINOGEN FRAGMENT D |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.540 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.192 |
| Total number of observations | 244386 * |
| Number of reflections | 40637 |
| Completeness [%] | 88.8 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 13-14% PEG 3350, 50 MM TRIS PH 7.0, 10 MM CALCIUM CHLORIDE 1MM SODIUM AZIDE 5MM GLY-PRO-ARG-PRO-AMIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 13-14 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | Gly-Pro-Arg-Pro-amide | 5 (mM) | |
| 5 | 1 | reservoir | 1 (mM) |
