1FYM

SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA IN A TAIL-TO-TAIL ORIENTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	106.510, 34.020, 78.210
Unit cell angles	90.00, 119.31, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.211 *
Rwork	0.211
R-free	0.29500
RMSD bond length	0.007
RMSD bond angle	1.388
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.049	0.186
Number of reflections	10644
<I/σ(I)>	12
Completeness [%]	84.5	72.7
Redundancy	3.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	4 *	PEG 4000, Isopropanol, Cacodylate, Ammonium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	1	Isopropanol
3	1	1	Cacodylate
4	1	1	Ammonium Acetate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	cacodylate	50 (mM)
4	1	reservoir	ammonium acetate	200 (mM)
5	1	drop	protein	0.91 (mM)
6	1	drop	DNA	0.69 (mM)
7	1	drop	Tris	10 (mM)
8	1	drop		50 (mM)
9	1	drop	ammonium acetate	400 (mM)