1FY7

CRYSTAL STRUCTURE OF YEAST ESA1 HISTONE ACETYLTRANSFERASE DOMAIN COMPLEXED WITH COENZYME A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2000-03-26
Detector	RIGAKU RAXIS IV
Spacegroup name	I 41 3 2
Unit cell lengths	181.500, 181.500, 181.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.216
Rwork	0.219
R-free	0.23600
RMSD bond length	0.006
RMSD bond angle	1.222
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.063	0.272
Total number of observations	1241495 *
Number of reflections	34558
<I/σ(I)>	48.6
Completeness [%]	99.9	99.8
Redundancy	35.9	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	amonium phosphate, cacodylate,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	CoA	0.5 (mM)
3	1	reservoir	sodium cacodylate	100 (mM)
4	1	reservoir	ammonium sulfate	1.7 (M)