1FXW

CRYSTAL STRUCTURE OF THE RECOMBINANT ALPHA1/ALPHA2 CATALYTIC HETERODIMER OF BOVINE BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	110
Detector technology	CCD
Collection date	1998-07-06
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 21
Unit cell lengths	44.519, 80.848, 116.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.218 *
Rwork	0.218
R-free	0.27700 *
RMSD bond length	0.009 *
RMSD bond angle	0.023 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.081 *	0.335 *
Total number of observations	273571 *
Number of reflections	25073 *
<I/σ(I)>	7.63
Completeness [%]	99.1 *	99.4 *
Redundancy	3.83	3.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.4	298	17% PEG-MME 2000, 100 mM Na acetate, pH 6.4, 10 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG2000 MME	17 (%)
2	1	1	sodium acetate	100 (mM)
3	1	1		10 (mM)
4	1	1	protein	12 (mg/ml)