1FXO
THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TMP COMPLEX.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-03 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 71.494, 73.064, 134.738 |
Unit cell angles | 89.93, 80.92, 81.11 |
Refinement procedure
Resolution | 73.000 - 1.660 |
R-factor | 0.145 |
Rwork | 0.142 |
R-free | 0.19600 |
Structure solution method | Phase extension in DM |
Starting model (for MR) | MAD structure (1G23) |
RMSD bond length | 0.016 |
RMSD bond angle | 2.320 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.120 | 1.740 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.053 | 0.332 |
Total number of observations | 1141819 * | |
Number of reflections | 297160 * | |
<I/σ(I)> | 10.9 | 2 |
Completeness [%] | 94.2 | 81.2 |
Redundancy | 3.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 * | Blankenfeldt, W., (2000) Acta Crystallogr.,Sect.D, 56, 1501. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 9-12 (%(w/v)) | |
2 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
3 | 1 | reservoir | citrate/NaOH | 0.1 (M) |