1FX0
Crystal structure of the chloroplast F1-ATPase from spinach
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-14 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.700, 147.700, 385.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 3.200 |
| R-factor | 0.319 * |
| Rwork | 0.319 |
| R-free | 0.35000 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.354 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.087 | 0.288 |
| Total number of observations | 231238 * | |
| Number of reflections | 23050 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 86.2 | 76 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 20 * | ammonium sulfate, HEPES, Na-azide, pH 7.5, Microbatch, temperature 25K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | HEPES | 25 (mM) | |
| 2 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 3 | 1 | 1 | PMSF | 0.002 (%(w/v)) | |
| 4 | 1 | 1 | 0.01 (%(w/v)) | ||
| 5 | 1 | 1 | ammonium sulfate | 1.5 (M) |
