1FWY

CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINE 1-PHOSPHATE URIDYLTRANSFERASE BOUND TO UDP-GLCNAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-03-12
Detector	ADSC QUANTUM 4
Spacegroup name	H 3 2
Unit cell lengths	140.780, 140.780, 244.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.217
R-free	0.25300
RMSD bond length	0.013
RMSD bond angle	24.700 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Rmerge	0.073	0.410 *
Number of reflections	40
<I/σ(I)>	5.1
Completeness [%]	97.3	98.1 *
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	20 *	1.3-1.5 M ammonium sulfate, 0.1 M MES, 4% acetone (v/v), pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	acetate	2 (mM)
3	1	reservoir	ammonium sulfate	1.3-1.5 (M)
4	1	reservoir	MES	0.1 (M)
5	1	reservoir	acetone	4 (%(v/v))