1FWK

CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-07-04
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	62.206, 129.147, 109.773
Unit cell angles	90.00, 105.52, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.209
R-free	0.24600
RMSD bond length	0.013 *
RMSD bond angle	1.690
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	0.026
Total number of observations	316441 *
Number of reflections	96677
<I/σ(I)>	29.5
Completeness [%]	99.4	99.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	20 *	PEG4000, sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
10	1	reservoir	Na acetate	0.2 (M)
2	1	drop	HEPES	50 (mM)
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	Tris-HCl	0.1 (M)
6	1	drop	Na acetate	0.2 (M)
7	1	drop	PEG4000	25 (%)
8	1	reservoir	PEG4000	20 (%)
9	1	reservoir	Tris-HCl	0.1 (M)