1FVI
CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.170, 58.440, 101.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.185 * |
| Rwork | 0.190 |
| R-free | 0.27000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.548 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.045 * |
| Completeness [%] | 98.6 * |
| Redundancy | 8.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | drop | protein | 18 (mg/ml) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) |
