1FVH
CRYSTAL STRUCTURE ANALYSIS OF NEURONAL SEC1 FROM THE SQUID L. PEALEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.125, 123.183, 63.891 |
| Unit cell angles | 90.00, 110.49, 90.00 |
Refinement procedure
| Resolution | 14.910 - 2.800 |
| R-factor | 0.234 * |
| Rwork | 0.234 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1epu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.699 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.054 | 0.287 |
| Number of reflections | 16849 | |
| <I/σ(I)> | 20.1 | 2.5 |
| Completeness [%] | 94.8 | 72.6 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | 277 | Bracher, A., (2000) Acta Crystallogr., Sect.D, 56, 501. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | 10 (mM) |
