1FUU

YEAST INITIATION FACTOR 4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 1
Unit cell lengths	38.800, 71.200, 73.200
Unit cell angles	94.00, 89.60, 101.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.252
Rwork	0.243
R-free	0.27300 *
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.071 *	0.097 *
Total number of observations	53337 *
Number of reflections	16117
Completeness [%]	92.0 *	80.4 *
Redundancy	2.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *	298	CHAPS, guanidinium, KOAc, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	mPEG550	20 (%)
2	1	1		1 (mM)
3	1	1	MES	100 (mM)