1FUI

L-FUCOSE ISOMERASE FROM ESCHERICHIA COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	127.300, 128.300, 239.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.162
Rwork	0.162
R-free	0.20900
Structure solution method	SINGLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.630
High resolution limit [Å]	2.500	2.500
Rmerge	0.099 *	0.297 *
Total number of observations	273560 *
Number of reflections	118481
<I/σ(I)>	6.5	2.4
Completeness [%]	87.0	82.4 *
Redundancy	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	2.01 M AMMONIUM SULFATE 2% PEG 400 1 MM MNCL2 10 MM 2-MERCAPTOETHANOL 10 MM L-FUCITOL 100 MM HEPES, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir		1 (mM)
11	1	reservoir	HEPES	0.1 (M)
12	1	reservoir	ammonium sulfate	2.01 (M)
2	1	drop	L-fucitol	10 (mM)
3	1	drop	ammonium sulfate	1.55 (M)
4	1	drop	PEG400	2 (%(w/v))
5	1	drop	beta-mercaptoethanol	10 (mM)
6	1	drop		1 (mM)
7	1	drop	HEPES	0.1 (M)
8	1	reservoir	PEG400	2 (%(w/v))
9	1	reservoir	beta-mercaptoethanol	10 (mM)