1FTC
Y13C MUTANT OF AZOTOBACTER VINELANDII FDI
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | XENTRONICS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.300, 73.700, 45.100 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.350 |
R-factor | 0.216 |
Rwork | 0.216 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.490 * |
Data reduction software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.350 * | 2.350 * |
Rmerge | 0.067 | |
Total number of observations | 25133 * | |
Number of reflections | 9115 * | |
Completeness [%] | 85.2 * | 32.9 * |
Redundancy | 2.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 12.5 (mM) | |
3 | 1 | drop | sodium citrate | 0.63 (M) | |
4 | 1 | drop | beta-mercaptoethanol | 0.25 (mM) | |
5 | 1 | reservoir | sodium citrate | 1.26 (M) | |
6 | 1 | reservoir | beta-mercaptoethanol | 0.5 (mM) |