1FTA

FRUCTOSE-1,6-BISPHOSPHATASE(D-FRUCTOSE-1,6-BISPHOSPHATE, 1-PHOSPHOHYDROLASE) (E.C.3.1.3.11) COMPLEXED WITH THE ALLOSTERIC INHIBITOR AMP

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	68.600, 84.800, 281.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.300
R-factor	0.2
Rwork	0.200
RMSD bond length	0.010
RMSD bond angle	2.570
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.099 *
Total number of observations	138799 *
Number of reflections	54785 *
Completeness [%]	74.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10-15 (mg/ml)
2	1	2	Tris base	50 (mM)
3	1	2		0.1 (mM)
4	1	2	citrate	0.1 (mM)
5	1	2	EDTA	0.1 (mM)
6	1	2		5 (mM)
7	1	2	2-mercaptoethanol	1.0 (mM)
8	1	2	PEG3350	7.5 (%(w/v))
9	1	2	AMP	0.1 (mM)