1FT9

STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	298
Detector technology	CCD
Collection date	1999-05-17
Detector	ADSC
Spacegroup name	P 41 21 2
Unit cell lengths	92.253, 92.253, 143.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.25
Rwork	0.230
R-free	0.28000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.800	0.400
Number of reflections	21881
<I/σ(I)>	12
Completeness [%]	98.7	99.1
Redundancy	5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.5	292	0.3 M MgCl2, 0.1 M Tris, 20% PEG 8000, pH 8.5, LIQUID DIFFUSION, temperature 292K
1	unknown *	8.5	292	0.3 M MgCl2, 0.1 M Tris, 20% PEG 8000, pH 8.5, LIQUID DIFFUSION, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	Tris-HCl	0.1 (M)
3	1	1		0.6 (M)
4	1	1	PEG8000	20 (%(v/v))
5	1	1	glycerol	20 (%(v/v))