1FSU

Crystal Structure of 4-Sulfatase (human)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	107.000, 107.000, 144.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9999.000 * - 2.500
R-factor	0.188
Rwork	0.188
R-free	0.24300
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	23.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.089 *
Total number of observations	194673 *
Number of reflections	27332
Completeness [%]	92.0	72.9
Redundancy	7.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.1		Ashby, S.J., (1995) Acta Cryst. D, 51, 1082. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	potassium phosphate	0.025 (M)
3	1	drop	PEG8000	10 (%)
4	1	reservoir	potassium phosphate	0.2 (M)
5	1	reservoir	PEG8000	14 (%)