1FSO

CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI QUADRUPLE MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	76.290, 76.290, 88.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.218
Rwork	0.217
R-free	0.23200
RMSD bond length	0.006
RMSD bond angle	1.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.029	0.300 *
Total number of observations	83504 *
Number of reflections	20565
<I/σ(I)>	13.7
Completeness [%]	99.7	99.7
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	293	ammonium sulfate and MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-15 (mg/ml)
2	1	reservoir	ammonium sulfate	44-48 (%sat)
3	1	reservoir	MES	100 (mM)