1FSK

COMPLEX FORMATION BETWEEN A FAB FRAGMENT OF A MONOCLONAL IGG ANTIBODY AND THE MAJOR ALLERGEN FROM BIRCH POLLEN BET V 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-05-21
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	91.653, 99.145, 108.905
Unit cell angles	105.70, 98.32, 97.62

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.253
Rwork	0.253
R-free	0.28500
RMSD bond length	0.010
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.047	0.370
Total number of observations	181008 *
Number of reflections	80241 *	6763 *
<I/σ(I)>	19.85
Completeness [%]	95.5	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4	277 *	12% peg 6000, 0.1M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	PEG6000	12 (%)
3	1	drop	sodium citrate	0.1 (M)
4	1	drop	sodium azide	0.01 (%)
5	1	reservoir	PEG6000	12 (%)
6	1	reservoir	sodium azide	0.01 (%)