1FSJ

CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-19
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	57.120, 82.960, 113.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
Rwork	0.189
R-free	0.22200
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.077	0.135
Number of reflections	49775
<I/σ(I)>	14.7
Completeness [%]	97.6	94.4
Redundancy	5.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	285	30% (w/v) PEG 5000, 0.1M sodium-acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K