1FSA
THE T-STATE STRUCTURE OF LYS 42 TO ALA MUTANT OF THE PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-02 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.050, 166.720, 79.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.870 * |
| Data reduction software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.090 |
| Total number of observations | 66515 * |
| Number of reflections | 27660 |
| <I/σ(I)> | 3.5 |
| Completeness [%] | 81.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | EDTA | 0.025 (mM) | |
| 4 | 1 | drop | PEG3500 | 7.5 (%) | |
| 5 | 1 | drop | 0.09 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | PEG3500 | 15 (%) |
