1FRW

STRUCTURE OF E. COLI MOBA WITH BOUND GTP AND MANGANESE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	95
Detector technology	CCD
Collection date	2000-07-19
Detector	ADSC QUANTUM 4
Spacegroup name	I 4 2 2
Unit cell lengths	124.387, 124.387, 67.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.750
R-factor	0.1856
Rwork	0.183
R-free	0.22600
RMSD bond length	0.022
RMSD bond angle	2.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.750	1.750
Rmerge	0.084	0.441
Number of reflections	26350
<I/σ(I)>	14
Completeness [%]	99.6	100
Redundancy	4.8	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	295	sodium acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	12 (mg/ml)
2	1	reservoir	sodium acetate	2.5-3 (M)
3	1	reservoir	cacodylic acid	0.1 (M)	pH6.5