1FQY

STRUCTURE OF AQUAPORIN-1 AT 3.8 A RESOLUTION BY ELECTRON CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	JEOL 3000 SFF
Temperature [K]	4
Detector technology	CCD
Collection date	1996-06-07
Detector	GATAN Model 795 MegaScan
Spacegroup name	P 4 21 2
Unit cell lengths	96.000, 96.000, 100.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.800
R-factor	0.406
Rwork	0.399
R-free	0.41700
RMSD bond length	0.017
RMSD bond angle	2.794
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	LATLINE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.000	3.900
High resolution limit [Å]	3.800	3.800
Rmerge	0.495	0.703
Number of reflections	85254
<I/σ(I)>	1.1
Completeness [%]	88.0	86.9
Redundancy	18.8	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Dialysis with continuous flow dialysis machine	6	298	Eletron Diffraction *