1FPT

THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN THE FAB FRAGMENT OF AN NEUTRALIZING ANTIBODY FOR TYPE 1 POLIOVIRUS AND ITS VIRAL EPITOPE

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	129.785, 129.785, 143.829
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.23
Rwork	0.230
RMSD bond length	0.018
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.099 *
Total number of observations	87269 *
Number of reflections	13342 *
Completeness [%]	86.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	20 (mg/ml)
2	1	drop	peptide	10 (mg/ml)
3	1	drop	Na citrate	1.1 (M)