1FOK
STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 1994-05-03 |
| Detector | SOL GRUNER, FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.590, 119.340, 71.520 |
| Unit cell angles | 90.00, 101.42, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.325 |
| Number of reflections | 26253 | |
| Completeness [%] | 98.5 | 96.1 |
| Redundancy | 7.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.15 (mM) | |
| 10 | 1 | drop | glycerol | 5 (%) | |
| 11 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 12 | 1 | reservoir | MES | 1.0 (M) | |
| 2 | 1 | drop | DNA | 0.75 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.1 (M) | |
| 4 | 1 | drop | MES | 0.5 (M) | |
| 5 | 1 | drop | 0.2 (M) | ||
| 6 | 1 | drop | potassium phosphate | 10 (mM) | |
| 7 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 8 | 1 | drop | EDTA | 0.5 (mM) | |
| 9 | 1 | drop | EGTA | 0.5 (mM) |
