1FNH

CRYSTAL STRUCTURE OF HEPARIN AND INTEGRIN BINDING SEGMENT OF HUMAN FIBRONECTIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MARRESEARCH
Wavelength(s)	.9787, .9788, .9115, .9796
Spacegroup name	I 2 2 2
Unit cell lengths	68.580, 86.290, 142.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.216 *
Rwork	0.216
R-free	0.29500
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	26.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.080	0.270
Number of reflections	5479
<I/σ(I)>	14	6
Completeness [%]	100.0	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	20 *	protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-6 (mg/ml)
2	1	reservoir	HEPES	0.1 (mM)
3	1	reservoir	Na, K Tartrate	0.8 (M)