1FNE
HISTOCOMPATIBILITY ANTIGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.900, 56.853, 116.726 |
| Unit cell angles | 90.00, 91.76, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.900 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iea |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.114 | 0.301 |
| Total number of observations | 364002 * | |
| Number of reflections | 72521 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 94.4 | 75.6 * |
| Redundancy | 5.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | 15% PEG 4000 15% 2-PROPANOL 300mM-500mM AMMONIUM ACETATE 100mM CITRATE PH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (OD280) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | sodium azide | 5 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 15 (%) | |
| 5 | 1 | reservoir | 2-propanol | 15 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 300-500 (mM) | |
| 7 | 1 | reservoir | citrate | 100 (mM) |
