1FN7
COUPLING OF DAMAGE RECOGNITION AND CATALYSIS BY A HUMAN BASE-EXCISION DNA REPAIR PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-07 |
| Detector | SBC-1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.113, 92.113, 211.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.850 - 2.600 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.540 |
| Rmerge | 0.073 | 0.352 |
| Total number of observations | 83683 * | |
| Number of reflections | 17653 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 96.6 | 99.8 |
| Redundancy | 4.74 | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | 100 mM sodium cacodylate pH 6.5, 200 mM calcium acetate, 16-18% PEG, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | PEG | ||
| 4 | 1 | 2 | PEG |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 6 | 1 | reservoir | calcium acetate | 200 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 16-18 (%) |
