1FMB
EIAV PROTEASE COMPLEXED WITH THE INHIBITOR HBY-793
Experimental procedure
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 42.930, 45.710, 56.770 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.136 * |
| Rwork | 0.136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HIV-1 PROTEASE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.046 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.067 |
| Total number of observations | 59519 * |
| Number of reflections | 8443 |
| Completeness [%] | 88.5 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EIAV PR | 3.6 (mg/ml) | |
| 2 | 1 | drop | urea | 6 (M) | |
| 3 | 1 | drop | potassium phosphate | 20 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | sodium acetate | 25 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 7 | 1 | reservoir | sodium azide | 0.1 (%) |
