1FLO
FLP Recombinase-Holliday Junction Complex I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-22 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.480, 180.180, 98.600 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.650 |
| R-factor | 0.249 * |
| Rwork | 0.249 |
| R-free | 0.29700 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.049 | 0.519 |
| Number of reflections | 75852 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 95.1 | 93 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | 12% PEG 5000 monomethyl ether, 20mM Hepes pH7.0, 10mM CaCl2, 100mM NaCl, 20% glycerol, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | PEG 5000 | ||
| 7 | 1 | 2 | PEG 5000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.1 (M) | ||
| 2 | 1 | reservoir | HEPES | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | 20 (%) | ||
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | PEG5000MME | 12 (%) | |
| 7 | 1 | drop | 0.1 (M) | ||
| 8 | 1 | drop | HEPES | 10 (mM) | |
| 9 | 1 | drop | dithiothreitol | 2 (mM) |
