1FL3
CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2000-03-31 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.930, 62.069, 93.539 |
| Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.1 |
| Rwork | 0.231 |
| R-free | 0.26200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.038 | 0.295 |
| Number of reflections | 36035 | |
| <I/σ(I)> | 29 | |
| Completeness [%] | 70 | |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 277 | PEG4000, magnesium chloride, sodium citrate, methionine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
