1FKM

CRYSTAL STRUCTURE OF THE YPT/RAB-GAP DOMAIN OF GYP1P

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-13
Detector	MARRESEARCH
Wavelength(s)	0.9790, 0.9795, 0.9200
Spacegroup name	P 65 2 2
Unit cell lengths	74.060, 74.060, 277.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.199
Rwork	0.199
R-free	0.22500
RMSD bond length	0.005
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.094	0.242
Total number of observations	237598 *
Number of reflections	33503
<I/σ(I)>	14.4
Completeness [%]	91.2	97.1
Redundancy	7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	PEG MME 5000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	PEG MME 5000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	5 (%)
2	1	reservoir	spermine-HCl	5-10 (mM)
3	1	reservoir	Tris	20 (mM)
4	1	drop	protein	10 (mg/ml)