1FKA
STRUCTURE OF FUNCTIONALLY ACTIVATED SMALL RIBOSOMAL SUBUNIT AT 3.3 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 406.300, 406.300, 173.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 3.300 |
| Rwork | 0.304 |
| R-free | 0.30500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.520 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.136 | 0.441 |
| Number of reflections | 206724 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 94.7 | 77.1 |
| Redundancy | 9.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 291 | Tsiboli, P., (1994) Eur. J. Biochem., 226, 169. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4Cl | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
