1FJ9
FRUCTOSE-1,6-BISPHOSPHATASE (MUTANT Y57W) PRODUCTS/ZN/AMP COMPLEX (T-STATE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 180 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-20 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.820, 165.480, 79.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.500 |
| Rwork | 0.194 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ieyj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.000 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 | 0.180 |
| Total number of observations | 88507 * | |
| Number of reflections | 25328 * | |
| <I/σ(I)> | 14.8 | 4 |
| Completeness [%] | 90.0 | 83 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 37 * | PEG3350, MPD, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 100 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | F6P | 5 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | AMP | 5 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 6 (%(w/v)) |
