1FIO
CRYSTAL STRUCTURE OF YEAST T-SNARE PROTEIN SSO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-14 |
| Detector | BRANDEIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.517, 49.855, 113.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26500 |
| Structure solution method | multiwavelength anomalous diffraction |
| RMSD bond length | 0.005 |
| RMSD bond angle | 16.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.074 | 0.380 |
| Number of reflections | 18088 | |
| <I/σ(I)> | 17.9 | 4.6 |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 4 | 1 | reservoir | isopropanol | 10 (%(v/v)) |
