1FIM
MACROPHAGE MIGRATION INHIBITORY FACTOR
Experimental procedure
| Temperature [K] | 283 |
| Collection date | 1995-05-18 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.800, 61.800, 53.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24400 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.886 * |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 5697 |
| Completeness [%] | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 18 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | reservoir | dioxane | 10 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
