1FH5
CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MONOCLONAL ANTIBODY MAK33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.910, 65.660, 73.460 |
| Unit cell angles | 109.14, 106.94, 95.91 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.061 | 0.279 |
| Number of reflections | 20143 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 93.7 | 87.1 * |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 4 * | na citrate, 16% PEG 8k, 16% glycerol 1.0 M NaCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 2 | 1 | reservoir | 1.0 (M) | ||
| 3 | 1 | reservoir | citrate | 50 (mM) | |
| 4 | 1 | reservoir | glycerol | 16 (%) |
