1FGU
SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-27 |
| Detector | SBC-2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.500, 84.860, 119.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.490 |
| Rmerge | 0.052 | 0.190 |
| Total number of observations | 247836 * | |
| Number of reflections | 22963 | |
| <I/σ(I)> | 37.1 | 4.6 |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 298 | 0.1 M K,NaH2PO4 0.1 M Tris 3.8 M NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 15 (mM) | |
| 3 | 1 | drop | 350 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | sodium potassium phospahte | 0.1 (M) | |
| 6 | 1 | reservoir | Tris | 0.1 (M) | |
| 7 | 1 | reservoir | 3.8 (M) |
