1FEU
CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 109.904, 109.904, 137.511 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.24500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 * |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.365 |
| Total number of observations | 188440 * | |
| Number of reflections | 41886 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 98.8 | 97.8 |
| Redundancy | 5.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | VAPOR DIFFUSION, HANGING DROP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2-4 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 4 (mM) | ||
| 5 | 1 | drop | MPD | 7 (%) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | MPD | 12 (%) |
