1FCY
ISOTYPE SELECTIVITY OF THE HUMAN RETINOIC ACID NUCLEAR RECEPTOR HRAR: THE COMPLEX WITH THE RARBETA/GAMMA-SELECTIVE RETINOID CD564
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.849, 59.849, 155.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.300 |
| R-factor | 0.133 * |
| Rwork | 0.133 |
| R-free | 0.16410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.031 | 0.413 |
| Total number of observations | 230315 * | |
| Number of reflections | 64898 | |
| <I/σ(I)> | 40.7 | 3 |
| Completeness [%] | 92.6 | 88.7 |
| Redundancy | 3.55 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 17 * | sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PIPES | 0.1 (M) | |
| 2 | 1 | reservoir | 0.2-1.7 (M) | ||
| 3 | 1 | drop | protein | 2-5 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | 1,4-dithio-DL-threitol | 5 (mM) | |
| 6 | 1 | drop | 500 (mM) | ||
| 7 | 1 | drop | n-dodecyl-alpha-D-maltoside | 0.15 (mM) | |
| 8 | 1 | drop | CHAPS | 2 (mM) | |
| 9 | 1 | drop | glycerol | 4 (%) |
