1FCP
FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1998-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 171.400, 171.400, 85.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 26.300 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.079 * | 0.406 * |
Number of reflections | 39633 | 4038 * |
<I/σ(I)> | 16.5 | 2.3 |
Completeness [%] | 98.9 | 98 |
Redundancy | 5.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.4 | 100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 3% PEG200 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.5 (mg/ml) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.4 |
3 | 1 | reservoir | PEG2000MME | 11 (%) | |
4 | 1 | reservoir | glycerol | 20 (%) | |
5 | 1 | reservoir | PEG200 | 3 (%) | |
6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
7 | 1 | reservoir | cis-inositol | 1 (%) |