1FCJ

CRYSTAL STRUCTURE OF OASS COMPLEXED WITH CHLORIDE AND SULFATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	96.380, 100.490, 142.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.2
Rwork	0.200
R-free	0.24700 *
RMSD bond length	0.011 *
RMSD bond angle	1.622 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.074 *	0.296 *
Number of reflections	84421 *
Completeness [%]	89.6 *	85.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir		0.18 (M)
5	1	reservoir	DMSO	5 (%(v/v))