1FCF
PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 102 |
| Detector technology | CCD |
| Collection date | 1999-04-08 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.753, 148.753, 100.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.31800 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.575 |
| Total number of observations | 224983 * | |
| Number of reflections | 25542 | |
| <I/σ(I)> | 30.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | ammonium sulfate, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | phenylboronic acid | 1 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 30-40 (%(v/v)sat) | |
| 5 | 1 | reservoir | 0.2-0.4 (M) |
