1FC3

THE CRYSTAL STRUCTURE OF TRANS-ACTIVATION DOMAIN OF THE SPORULATION RESPONSE REGULATOR, SPO0A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Collection date	1999-12-10
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	43.118, 53.414, 53.718
Unit cell angles	90.82, 111.73, 111.32

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.1991
Rwork	0.196
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.060 *	0.313 *
Number of reflections	27976
<I/σ(I)>	19.41	3.23
Completeness [%]	87.6	55.6
Redundancy	3.6 *	1.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	289 *	Muchova, K., (1999) Acta Crystallogr., D55, 671. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	PEG4000	10 (%(w/v))
5	1	reservoir		50 (mM)
6	1	reservoir	Tris-HCl	50 (mM)