1FBN
CRYSTAL STRUCTURE OF A FIBRILLARIN HOMOLOGUE FROM METHANOCOCCUS JANNASCHII, A HYPERTHERMOPHILE, AT 1.6 A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 170 |
| Collection date | 1997-10-01 |
| Wavelength(s) | 0.97921,0.97894,0.96373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.407, 43.264, 55.303 |
| Unit cell angles | 90.00, 96.99, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.600 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.24800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 * | |
| Number of reflections | 37155 | |
| Completeness [%] | 97.0 | 95.8 |
| Redundancy | 2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 20% 2-PROPANOL, 20% PEG4k, 0.1M SODIUM CITRATE, pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 10 (%) | |
| 3 | 1 | drop | isopropanol | 10 (%) | |
| 4 | 1 | drop | sodium citrate | 0.05 (M) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%) | |
| 6 | 1 | reservoir | isopropanol | 20 (%) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) |
