1F9W

CRYSTAL STRUCTURES OF MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE KINESIN MOTOR ATPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-21
Spacegroup name	P 43
Unit cell lengths	62.890, 62.890, 153.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.23 *
Rwork	0.230
R-free	0.27630
RMSD bond length	0.009
RMSD bond angle	1.420
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.085
Total number of observations	265649 *
Number of reflections	20874
<I/σ(I)>	18.8
Completeness [%]	96.0	66.9
Redundancy	12.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	HEPES, PEG 2000 ME, Etylen Glycol,Magnesium chloride, Sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG2000 ME	20-26 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	HEPES	50 (mM)