1F9T
CRYSTAL STRUCTURES OF KINESIN MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE MOTOR ATPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.610, 78.810, 47.170 |
| Unit cell angles | 90.00, 105.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.24400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.199 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.074 | |
| Total number of observations | 793427 * | |
| Number of reflections | 47889 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 97.3 | 94.9 |
| Redundancy | 16.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | HEPES, PEG 2000 ME, magnesium chloride, Ethylen glycol, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 ME | 20-26 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | HEPES | 50 (mM) |
