1F8Y

CRYSTAL STRUCTURE ANALYSIS OF NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE COMPLEXED WITH 5-METHYL-2'-DEOXYPSEUDOURIDINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	296
Detector technology	AREA DETECTOR
Collection date	1992-01-01
Detector	SDMS
Spacegroup name	I 21 3
Unit cell lengths	149.600, 149.600, 149.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.400
R-factor	0.16
Rwork	0.160
R-free	0.21800
RMSD bond length	0.008
RMSD bond angle	1.560
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.400
Rmerge	0.075
Total number of observations	101311 *
Number of reflections	21897
Completeness [%]	93.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	296	Ammonium Sulfate, Citrate Buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	ammonium sulfate	5 (%sat)
3	1	drop	citrate	0.05 (M)
4	1	reservoir	ammonium sulfate	5 (%sat)
5	1	reservoir	citrate	0.05 (M)