1F8N

LIPOXYGENASE-1 (SOYBEAN) AT 100K, NEW REFINEMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1994-02-01
Detector	CUSTOM-MADE
Spacegroup name	P 1 21 1
Unit cell lengths	94.900, 94.000, 49.900
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.198 *
Rwork	0.198
R-free	0.21200
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.037	0.343
Number of reflections	161029
<I/σ(I)>	35.7
Completeness [%]	96.0	91.6
Redundancy	6.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6 *	21 *	Minor, W., (1996) Biochemistry, 35, 10687. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	acetate	0.1 (M)
2	1	drop	PEG3400	4 (%(w/v))
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	PEG3350	9 (%)
5	1	reservoir	sodium acetate	0.2 (M)