1F7C

CRYSTAL STRUCTURE OF THE BH DOMAIN FROM GRAF, THE GTPASE REGULATOR ASSOCIATED WITH FOCAL ADHESION KINASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-04-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	65.026, 65.026, 91.624
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.205
R-free	0.25200
Structure solution method	MIR and MR
RMSD bond length	0.006
RMSD bond angle	19.400 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.077	0.330 *
Total number of observations	30884 *
Number of reflections	9020 *
<I/σ(I)>	11.4
Completeness [%]	99.0	98 *
Redundancy	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	294	PEG 6000, Na HEPES, pH 7.0, VAPOR DIFFUSION, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG6000	20-22 (%)
3	1	reservoir	HEPES	100 (mM)