1F6B

CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	1999-02-13
Spacegroup name	P 1 21 1
Unit cell lengths	53.368, 61.689, 71.142
Unit cell angles	90.00, 107.49, 90.00

Refinement procedure

Resolution	15.000 * - 1.700
R-factor	0.22
Rwork	0.220
R-free	0.24000
RMSD bond length	0.006 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.050	0.260
Number of reflections	48000
<I/σ(I)>	24.5
Completeness [%]	98.8	88.8
Redundancy	8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295.5	30% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
1	VAPOR DIFFUSION, SITTING DROP	4.6	295.5	30% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
1	VAPOR DIFFUSION, SITTING DROP	4.6	295.5	30% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
1	VAPOR DIFFUSION, SITTING DROP	4.6	295.5	30% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K